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KMID : 0624620170500090478
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BMB Reports
2017 Volume.50 No. 9 p.478 ~ p.483
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Heterogeneous interaction network of yeast prions and remodeling factors detected in live cells
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Pack Chan-Gi
Inoue Yuji
Higurashi Takashi
Kawai-Noma Shigeko
Hayashi Daigo
Craig Elizabeth
Taguchi Hideki
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Abstract
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Budding yeast has dozens of prions, which are mutually dependent on each other for the de novo prion formation. In addition to the interactions among prions, transmissions of prions are strictly dependent on two chaperone systems: the Hsp104 and the Hsp70/Hsp40 (J-protein) systems, both of which cooperatively remodel the prion aggregates to ensure the multiplication of prion entities. Since it has been postulated that prions and the remodeling factors constitute complex networks in cells, a quantitative approach to describe the interactions in live cells would be required. Here, the researchers applied dual-color fluorescence cross-correlation spectroscopy to investigate the molecular network of interaction in single live cells. The findings demonstrate that yeast prions and remodeling factors constitute a network through heterogeneous protein-protein interactions.
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KEYWORD
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Fluorescence cross-correlation spectroscopy, Live cell, Protein interaction, Remodeling factors, Yeast prions
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